Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

5.9K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.9K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.3K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.3K
Autophagic Cell Death01:18

Autophagic Cell Death

4.7K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
4.7K
Replicative Cell Senescence02:15

Replicative Cell Senescence

4.5K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.5K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K
Mitochondria01:37

Mitochondria

20.8K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
20.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Omnidirectional 3D Printing of Anisotropic Nanofibrous Peptide Hydrogels.

bioRxiv : the preprint server for biology·2025
Same author

Autophagy in Tissue Repair and Regeneration.

Cells·2025
Same author

Targeting CRM1 for Progeria Syndrome Therapy.

Aging cell·2025
Same author

Effect of the Ketone Body, D-β-Hydroxybutyrate, on Sirtuin2-Mediated Regulation of Mitochondrial Quality Control and the Autophagy-Lysosomal Pathway.

Cells·2023
Same author

Nucleophagy contributes to genome stability through degradation of type II topoisomerases A and B and nucleolar components.

Journal of cell science·2023
Same author

Chromatin Structure from Development to Ageing.

Sub-cellular biochemistry·2023

Related Experiment Video

Updated: Feb 19, 2026

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

6.4K

Connecting chaperone-mediated autophagy dysfunction to cellular senescence.

Daniel Moreno-Blas1, Elisa Gorostieta-Salas1, Susana Castro-Obregón1

  • 1Department of Neurodevelopment and Physiology, Institute of Cellular Physiology, National Autonomous University of México (UNAM), Mexico City, Mexico.

Ageing Research Reviews
|November 9, 2017
PubMed
Summary

Impaired chaperone-mediated autophagy (CMA) function in aging leads to protein buildup and cellular senescence. Loss of CMA activity during aging promotes cellular senescence, contributing to age-related diseases.

Keywords:
AgingCellular senescenceChaperone-mediated autophagyMacroautophagyProteostasis

More Related Videos

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

6.8K
Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.2K

Related Experiment Videos

Last Updated: Feb 19, 2026

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

6.4K
A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

6.8K
Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.2K

Area of Science:

  • Cellular Biology
  • Aging Research
  • Molecular Biology

Background:

  • Chaperone-mediated autophagy (CMA) is a key lysosome-autophagy pathway for selective protein degradation.
  • Aging impairs CMA, leading to accumulation of damaged proteins and inefficient stress responses.
  • Accumulated proteins disrupt proteostasis, activating pathways that induce cellular senescence.

Purpose of the Study:

  • To review and integrate evidence on the relationship between CMA activity and cellular senescence.
  • To investigate the role of CMA dysfunction in aging and age-related diseases.
  • To propose that impaired CMA promotes cellular senescence during aging.

Main Methods:

  • Literature review and evidence integration.
  • Analysis of cellular proteostasis mechanisms.
  • Examination of signaling pathways involved in senescence.

Main Results:

  • CMA dysfunction correlates with hallmarks of cellular senescence.
  • Impaired protein degradation due to CMA loss contributes to cellular aging.
  • Evidence suggests CMA deficiency is linked to age-related disease pathology.

Conclusions:

  • Loss of CMA function during aging promotes cellular senescence.
  • CMA impairment is a significant factor in age-related cellular dysfunction.
  • Restoring CMA function may offer therapeutic strategies for aging and related diseases.