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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

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Related Experiment Video

Updated: Feb 19, 2026

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Phenotypic Screen Identifies a Small Molecule Modulating ERK2 and Promoting Stem Cell Proliferation.

Chang Yin^1,2, Temesgen Fufa^1,3, Gayathri Chandrasekar^1,4

¹Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Frontiers in Pharmacology

|November 9, 2017

Summary

Researchers identified a small molecule that enhances stem cell proliferation using integrated phenotypic drug discovery (PDD) models. This approach combines in vitro and in vivo methods for improved hit identification and reproducibility in drug discovery.

Keywords:

PDD mouse phenotype small molecules stem cells zebrafish

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Identification of Kinase-substrate Pairs Using High Throughput Screening

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Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Area of Science:

Stem cell biology
Pharmacology
Drug discovery

Background:

Stem cells possess unique proliferation controls distinct from somatic cells.
Understanding these mechanisms is crucial for advancing drug discovery and translational applications.
Phenotype-based drug discovery (PDD) offers an unbiased route to novel biological insights.

Purpose of the Study:

To identify small molecules that promote stem cell proliferation.
To evaluate an integrated phenotypic screening approach using combined in vitro and in vivo models.
To characterize the mechanism of action for identified compounds.

Main Methods:

Employed an integrated phenotypic screening approach combining in vitro and in vivo PDD models.
Utilized cell viability and colony size measurements for phenotype assessment.
Performed structure-activity relationship studies on the lead molecule.

Main Results:

The integrated PDD approach improved hit identification and reproducibility across models.
A novel small molecule was identified that significantly increases stem cell proliferation.
The molecule was found to inhibit ERK2 phosphorylation, a key pathway in cell proliferation.

Conclusions:

An integrated PDD strategy using combinatorial models effectively identifies compounds promoting stem cell proliferation.
This approach enhances the discovery of molecules with potential therapeutic applications in regenerative medicine.
The identified small molecule targeting ERK2 phosphorylation represents a promising candidate for further investigation.