Spectroscopic determination of succinylcholine in dosage forms using eosin Y

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Succinylcholine chloride (SUC) is a critical neuromuscular blocking agent.
• Accurate quantification of SUC in pharmaceutical preparations is essential for quality control.

Purpose of the Study:

• To develop simple, sensitive analytical methods for SUC estimation.
• To validate these methods for pharmaceutical preparations.

Main Methods:

• Spectrophotometry: Ion pair complex formation with eosin Y, measured at 550 nm.
• Spectrofluorimetry: Quenching of eosin Y native fluorescence by SUC, measured at 550 nm (excitation 480 nm).
• Both methods employed Britton Robinson buffer at pH 3.

Main Results:

• Spectrophotometry linearity: 0.5–15 μg/ml SUC.
• Spectrofluorimetry linearity: 0.05–1 μg/ml SUC.
• Successful application for succinylcholine ampoule estimation.

Conclusions:

• Developed spectrophotometric and spectrofluorimetric methods are simple, sensitive, and suitable for SUC quantification.
• The methods provide reliable results for pharmaceutical quality control.
• Reaction pathway elucidation supports the analytical basis.