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SIRT5 promotes cell proliferation and invasion in hepatocellular carcinoma by targeting E2F1

Liang Chang1, Liang Xi2, Yubin Liu3

  • 1Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Molecular Medicine Reports
|November 9, 2017
PubMed

Insights

Sirtuin 5 (SIRT5) is upregulated in hepatocellular carcinoma (HCC), promoting cancer cell proliferation and invasion. Inhibiting SIRT5 may offer a new therapeutic strategy for liver cancer.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Sirtuin 5 (SIRT5) is an NAD+-dependent deacetylase involved in liver metabolism.
  • Its precise role in hepatocellular carcinoma (HCC) proliferation is not fully understood.

Purpose of the Study:

  • To investigate the expression and function of SIRT5 in HCC.
  • To elucidate the mechanism by which SIRT5 influences HCC progression.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis for expression.
  • Chromatin immunoprecipitation (ChIP) and luciferase reporter assays for mechanism.
  • In vitro proliferation and Transwell assays for functional analysis.

Main Results:

  • SIRT5 was upregulated in HCC tissues and cell lines, correlating with poorer survival.
  • SIRT5 inhibition suppressed HCC cell proliferation and invasion; overexpression promoted them.
  • E2F transcription factor 1 (E2F1) was identified as a novel SIRT5 target, mediating its effects on proliferation and invasion.

Conclusions:

  • SIRT5 plays a significant role in promoting HCC cell proliferation and invasion.
  • SIRT5 acts via regulating E2F1 expression.
  • SIRT5 represents a potential therapeutic target for HCC.

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