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Updated: Feb 19, 2026
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
Published on: March 31, 2018
miR‑126 is essential for endothelial phenotype expression during endothelial differentiation in adipose‑derived stem
Han Xie1, Fang Wang2, Xiao Chen1
1Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, P.R. China.
Abstract:
The endothelial differentiation of stem cells serves an essential role in vascular development, function and disease. Autograft adipose‑derived stem cells (ADSCs) may be a novel source of cell for use in the study of angiogenesis. microRNA‑126 (miR‑126) has been extensively studied in endothelial cells. However, the effect of miR‑126 on the endothelial differentiation of ADSCs has not been demonstrated. In the present study, it was observed that the expression of miR‑126 was markedly increased during the endothelial differentiation of ADSCs in a time‑dependent manner. The effect of miR‑126 on ADSC differentiation was confirmed by employing up‑ and down‑regulation strategies. The expression of endothelial markers was decreased by miR‑126 inhibitor transfection during endothelial differentiation. The results of the present study suggest that miR‑126 is essential for endothelial phenotype expression, and may promote the progress of endothelial differentiation in ADSCs, providing a novel strategy for modulating vascular formation and function.
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