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Published on: October 19, 2013
Attenuated SUMOylation of sirtuin 1 in premature neonates with bronchopulmonary dysplasia
Fengmei Tan1, Wenbin Dong1, Xiaoping Lei1
1Department of Neonatology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
Abstract:
A prospective study was performed to investigate the effects of hyperoxia on the expression of small ubiquitin‑related modifier (SUMO) and sirtuin 1 (SIRT1) proteins, and to examine interactions between these proteins in premature neonates with bronchopulmonary dysplasia (BPD). Peripheral blood mononuclear cells (PBMCs) were isolated from residual venous blood samples of 20 premature infants with BPD and 20 gender‑matched premature infants without BPD (non‑BPD group). Expression levels of SUMO and SIRT1 proteins in PBMCs were assessed by western blot analysis, and their interactions in PBMCs were detected using the immunoprecipitation assay. Based on the fraction of inspired oxygen (FiO2) administered, neonates were divided into normoxia, low‑(21%

