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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Identification of differentially expressed microRNAs in acute Kawasaki disease
Ye Chen1, Yue-Yue Ding1, Yan Ren2
1Cardiology Department, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China.
Abstract:
The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in an independent cohort (n=8). Target genes of the differentially expressed miRNAs were predicted and analyzed for gene ontology term enrichment and Kyoto Encyclopedia of Genes and Genomes pathways. miRNA microarray analysis revealed that seven miRNAs (miRs) were significantly upregulated (hsa‑let‑7b‑5p, hsa‑miR‑223‑3p, hsa‑miR‑4485, hsa‑miR‑4644, hsa‑miR‑4800‑5p, hsa‑miR‑6510‑5p and hsa‑miR‑765) and three were significantly downregulated (hsa‑miR‑33b‑3p, hsa‑miR‑4443 and hsa‑miR‑4515) in acute KD compared with the healthy controls. hsa‑miR‑223‑3p expression levels detected by RT‑qPCR were consistent with the microarray results. A total of 62 target genes of hsa‑miR‑223‑3p were predicted. In total, 10 differentially expressed miRNAs were identified in acute KD, of which hsa‑miR‑223‑3p was verified by RT‑qPCR.
Insights
This study identified 10 differentially expressed microRNAs (miRNAs) in children with Kawasaki disease (KD). Hsa-miR-223-3p was significantly upregulated and validated, offering insights into KD pathogenesis.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Pediatric Immunology
Background:
- Kawasaki disease (KD) is an acute febrile vasculitis affecting young children.
- The precise pathogenesis of KD remains incompletely understood.
- MicroRNAs (miRNAs) are implicated in various inflammatory and immune processes.
Purpose of the Study:
- To screen plasma microRNA expression in acute Kawasaki disease (KD) patients.
- To identify differentially expressed miRNAs that may contribute to KD pathogenesis.
- To validate key miRNA findings and explore their potential targets.
Main Methods:
- Plasma samples from children with acute KD (n=6) and healthy controls (n=6) were analyzed using miRNA microarray.
- Differential miRNA expression was confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in an independent cohort (n=8).
- Target genes of identified miRNAs were predicted and analyzed for pathway enrichment.
Main Results:
- Microarray analysis revealed 10 significantly differentially expressed miRNAs in acute KD patients compared to controls.
- Seven miRNAs were upregulated, including hsa-let-7b-5p and hsa-miR-223-3p.
- Three miRNAs were downregulated, and hsa-miR-223-3p expression was validated by RT-qPCR.
Conclusions:
- This study identified a panel of 10 differentially expressed miRNAs in acute Kawasaki disease.
- Hsa-miR-223-3p is a potential biomarker and therapeutic target for KD.
- Further research into miRNA-mediated pathways could elucidate KD pathogenesis.
