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Updated: Feb 19, 2026

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
Published on: March 11, 2021
A new tetraphenylethylene based AIE sensor with light-up and tunable measuring range for adenosine triphosphate in
Guoyu Jiang1, Wenping Zhu, Qingqing Chen
1Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, P. R. China. wangjg@iccas.ac.cn.
Abstract:
An AIE based tetraphenylethylene derivative (TPPTPE) was synthesized for light-up sensing of ATP in aqueous solution. The measuring range for ATP can be tuned by varying the concentration of the TPPTPE. A one-step straightforward quantitative analysis of the ATP level in cell lysates can be realized using the TPPTPE. Moreover, the TPPTPE can be used for monitoring apyrase activity in aqueous solution and detecting ATP both in living cancer cell lines and in living normal cell lines.

