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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Feb 19, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Continuous-flow, microfluidic, qRT-PCR system for RNA virus detection.

B Leticia Fernández-Carballo^1,2, Christine McBeth¹, Ian McGuiness¹

¹Fraunhofer USA - Center for Manufacturing Innovation, 15 Saint Mary's Street, Brookline, MA, 02446, USA.

Analytical and Bioanalytical Chemistry

|November 9, 2017

Summary

This summary is machine-generated.

This study presents a rapid, microfluidic reverse transcription polymerase chain reaction (RT-PCR) system for detecting RNA viruses like Ebola. The point-of-care platform offers fast and accurate infectious disease diagnosis.

Keywords:

Ebola virus Infectious diseases Lab on a chip Point of care Quantitative reverse transcription polymerase chain reaction RNA-based virus detection

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Last Updated: Feb 19, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Published on: November 10, 2023

Area of Science:

Biomedical Engineering
Molecular Biology
Infectious Disease Diagnostics

Background:

Rapid and accurate pathogen detection is crucial for managing infectious diseases and preventing the spread of drug-resistant pathogens.
Point-of-care (POC) reverse transcription polymerase chain reaction (RT-PCR) platforms are of significant interest for detecting RNA viruses.
Current diagnostic methods face challenges in speed and accessibility in various settings.

Purpose of the Study:

To develop a microfluidic, real-time, fluorescence-based, continuous-flow RT-PCR system for rapid pathogen detection.
To create a cost-effective, industrially scalable disposable microfluidic chip for the developed system.
To demonstrate the system's efficacy and determine its performance characteristics for RNA virus detection.

Main Methods:

Development of a disposable thermoplastic microfluidic chip using roll-to-roll embossing.
Integration of a continuous-flow system with distinct zones for reverse transcription and thermal cycling (amplification and real-time detection).
Testing the system for Ebola virus detection, evaluating different master mixes, limit of detection, and amplification speed.

Main Results:

Successful development of a microfluidic continuous-flow RT-PCR system.
Demonstrated proof-of-concept for Ebola virus detection.
Characterization of the system's limit of detection and maximum amplification speed.

Conclusions:

The developed microfluidic RT-PCR system offers a promising solution for rapid and accurate point-of-care infectious disease diagnosis.
The system's design is versatile and suitable for detecting various RNA-based viruses, including Zika and chikungunya.
Industrial scalability and cost-effectiveness are key advantages for global health applications.