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Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format

Ulrike S Diesterbeck1

  • 1Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 33 North Drive, Bethesda, MD, 20892, USA. ulrike.diesterbeck@nih.gov.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2017
PubMed

Recombinant immunoglobulins are an excellent tool for diagnosis, treatment, and passive immunization. Phage display offers a robust technique for the selection of recombinant antibodies from immunoglobulin libraries. The construction of immunoglobulin libraries for veterinary purposes was restricted by the lack of knowledge about species-specific diversities.The now available data enable the construction of highly diverse libraries in livestock like cattle. Using diverse primer sets, the immunoglobulin repertoire is amplified and ligated into a phagemid. Infection of E. coli with filamentous phages allows the display of the immunoglobulin fragments on the surface as a fusion protein to the phage's minor coat protein 3.

Keywords:
Bos taurusBovine immunoglobulinsBovine scFvHeavy chain variable regionImmunoglobulin librariesLight chain variable region

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