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Updated: Feb 19, 2026
IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol PEG Precipitation
Published on: May 1, 2011
Construction of Chicken Antibody Libraries
Jeanni Fehrsen1, Susan Wemmer2, Wouter van Wyngaardt2
1Immunology Division, New Generation Vaccines Programme, ARC-Onderstepoort Veterinary Research, Onderstepoort, Pretoria, 0110, South Africa. fehrsenj@arc.agric.za.
Abstract:
Recombinant antibody libraries based on chicken immunoglobulin genes are potentially valuable sources of phage displayed scFvs for use in veterinary diagnostics and research. The libraries described in this chapter are based on chicken variable heavy and light chain immunoglobulin genes joined by a short flexible linker cloned in the phagemid vector pHEN1. The resulting phagemids produce either scFvs displayed on the surface of the fusion phage subsequent to co-infection with helper phage, or soluble scFvs following IPTG induction. This chapter provides detailed and proven methods for the construction of such libraries.
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