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Metasecretome Phage Display.
Milica Ciric1, Filomena Ng1,2, Jasna Rakonjac1
1Institute of Fundamental Sciences, Massey University, Private Bag 11 222, Palmerston North, 4442, New Zealand.
Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2017
Summary
This study introduces metasecretome phage display, a novel method to analyze microbial cell-surface and secreted proteins. This technique overcomes limitations of traditional methods by enabling direct study of microbial community diversity.
Area of Science:
- Microbiology
- Molecular Biology
- Bioinformatics
Background:
- The metasecretome comprises proteins mediating microbial community-environment interactions.
- Traditional cultivation methods limit the exploration of microbial diversity.
- Metagenomics offers culture-independent analysis of microbial genomes.
Purpose of the Study:
- To present a protocol for metasecretome phage display.
- To enable comprehensive analysis of microbial community secretomes.
- To facilitate functional screening of secreted proteins.
Main Methods:
- Development of the metasecretome phage display protocol.
- Selective display of the metasecretome from metagenomic data.
- Application of PacBio sequencing for whole metasecretome analysis.
- Affinity-screening (bio-panning) for functional protein identification.
Main Results:
- A method to selectively display the metasecretome is established.
- The metasecretome library allows for complete sequencing.
- Functional screening of specific metasecretome proteins is enabled.
Conclusions:
- Metasecretome phage display is a powerful tool for studying microbial communities.
- This method enhances the understanding of microbial interactions and functions.
- It provides a platform for discovering novel proteins with specific functions.
