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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers.

Yuqian Gao¹, Hui Wang¹, Carrie D Nicora¹

¹Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 9, 2017

Summary

Liquid chromatography-selected reaction monitoring (LC-SRM) offers sensitive and reproducible protein quantification. This method accurately measures protein biomarkers in human urine using stable isotope-labeled standards.

Keywords:

Biomarker LC-SRM Skyline Stable heavy isotope-labeled peptide Targeted quantification Urine

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Last Updated: Feb 19, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

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Published on: August 17, 2015

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Published on: May 8, 2021

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Published on: December 8, 2023

Area of Science:

Biochemistry
Analytical Chemistry
Proteomics

Background:

Liquid chromatography-selected reaction monitoring (LC-SRM) is a highly sensitive, reproducible, and multiplexing technique for protein quantification.
Accurate measurement of targeted proteins in complex biological samples is crucial for biomarker discovery.
Stable heavy isotope-labeled peptides serve as effective internal standards for precise quantification.

Purpose of the Study:

To provide a detailed protocol for applying LC-SRM.
To enable the quantification of candidate protein biomarkers in human urine.

Main Methods:

Utilizing liquid chromatography coupled with selected reaction monitoring (LC-SRM).
Employing stable heavy isotope-labeled peptides as internal standards.
Quantifying surrogate proteotypic peptides for targeted proteins in human urine samples.

Main Results:

Demonstrated the successful application of LC-SRM for protein biomarker quantification.
Established a step-by-step protocol for reproducible results.
Validated the accuracy of LC-SRM in complex human urine matrices.

Conclusions:

LC-SRM is a powerful and accurate method for quantifying protein biomarkers in human urine.
The developed protocol facilitates the application of LC-SRM in clinical and research settings.
This technique supports biomarker discovery and validation efforts.