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A rapid analysis of nafcillin using high-performance liquid chromatography
1College of Pharmacy, Ohio State University, Columbus 43210.
Therapeutic Drug Monitoring
|January 1, 1989
Summary
A new high-performance liquid chromatography method accurately measures nafcillin in serum. This simple, rapid assay uses a single-step extraction and a small sample volume, making it ideal for clinical applications.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Accurate measurement of nafcillin serum concentrations is crucial for effective patient treatment.
- Existing methods for nafcillin quantification may be complex or time-consuming.
Purpose of the Study:
- To develop and validate a simple, rapid, and reproducible high-performance liquid chromatography (HPLC) method for nafcillin measurement in serum.
- To establish the method's accuracy, precision, and efficiency for clinical use.
Main Methods:
- A one-step protein precipitation was employed for sample preparation, utilizing nafcillin and an internal standard (IS), 5-(p-hydroxyphenyl)-5-phenylhydantoin.
- The HPLC analysis used an Ultrasphere ODS reversed-phase column with a mobile phase of 0.02 M ammonium acetate buffered solution and acetonitrile (75:25 vol/vol), with detection at 224 nm.
Main Results:
- The method demonstrated high absolute recovery for nafcillin (95-98%).
- Intraday and interday coefficients of variation were low (3.5% and 3.1-7.1%, respectively), indicating good precision.
- The assay required only a small serum volume (50 microliters) and featured a single-step extraction.
Conclusions:
- The developed HPLC method is a simple, rapid, and reproducible technique for determining nafcillin serum concentrations.
- Its efficiency and minimal sample requirement make it suitable for routine clinical monitoring, particularly in pediatric patients.