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Bacteriophage Production in Bioreactors.

Maryam Agboluaje1, Dominic Sauvageau2

  • 1Chemical and Materials Engineering, University of Alberta, 12th floor Donadeo ICE Building, 9211 116 St NW, Edmonton, AB, Canada, T5M 0L5.

Methods in Molecular Biology (Clifton, N.J.)
|November 10, 2017
PubMed
Summary

This study outlines a versatile method for producing virulent bacteriophages in bioreactors, adaptable to various systems and conditions. It details optimizing infection parameters and operational procedures for efficient bacteriophage yield.

Keywords:
Bacteriophage productionBatch reactorsBioreactorsOptimizationTwo-stage production

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Area of Science:

  • Microbiology
  • Biotechnology
  • Bioprocess Engineering

Background:

  • Bacteriophage production in bioreactors requires specific conditions that vary by host-phage system.
  • A standardized, adaptable method is needed for consistent virulent bacteriophage production.

Purpose of the Study:

  • To present a general, adaptable method for producing virulent bacteriophages in bioreactors.
  • To provide detailed procedures for optimizing various parameters in bacteriophage production.

Main Methods:

  • Developing a generalizable protocol for virulent bacteriophage production in bioreactors.
  • Detailing host pre-culture and bioreactor preparation.
  • Establishing optimal initial infection conditions (infection load and initial multiplicity of infection (MOI)).
  • Outlining bioreactor operation and bacteriophage harvesting procedures.
  • Discussing batch, two-stage continuous, and two-stage cycling bioreactor modes.

Main Results:

  • A comprehensive method for virulent bacteriophage production is presented.
  • The method is adaptable to diverse host-bacteriophage systems and operating conditions.
  • Optimization of infection load and MOI is crucial for efficient production.

Conclusions:

  • The presented general method facilitates adaptable and efficient virulent bacteriophage production in bioreactors.
  • This approach supports various bioprocess configurations, including batch and continuous modes.
  • Optimized conditions ensure consistent and high-yield bacteriophage harvests.