Related Experiment Video
Updated: Feb 19, 2026
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
Published on: November 17, 2023
Rapid and sensitive detection of pyrimethanil residues on pome fruits by Surface Enhanced Raman Scattering
L Mandrile1, A M Giovannozzi2, F Durbiano2
1Division of Metrology for Quality of Life, Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce, 91 10135 Torino, Italy; Department of Chemistry and Interdepartmental Centre NIS, University of Turin, Via Giuria 7, 10125 Turin, Italy.
Abstract:
Surface Enhanced Raman Scattering (SERS) supported by gold nanoparticles (AuNPs) was applied to detect and quantify residues of pyrimethanil on pome fruits, a widely used fungicide in horticultural species. Spheroidal AuNPs with different size were fabricated and compared in this study. The analytical procedure was set up on a silicon dioxide flat substrate to standardize SERS methodology. A Raman mapping strategy was exploited to increase signal reproducibility and to minimize bias due to different local surface morphologies. Univariate and multivariate regressions were compared for calibration. Multivariate PLS approach demonstrated acceptable repeatability and method stability (RMSECV = 4.79 ppm; RMSEP = 4.31 ppm) in the range 0-40 mg kg-1, providing higher accuracy and intra-day repeatability with a mean percentage error of 18.7% and 32.8% for PLS and univariate calibration, respectively. The method here proposed can be reliably applied for PMT detection on pome fruits within the European law limits.
