Effect of 1-methyl-3-hexylimidazolium bromide on zebrafish (Danio rerio)

Room-temperature ionic liquids, generally referred to ionic liquids (ILs), are "green solvents". Antioxidant responses and DNA damage in zebrafish livers exposed to 1-methyl-3-hexylimidazolium bromide ([C₆mim]Br) were evaluated at various doses (5-40 mg/L) for a 28-day IL-exposure. A significant decrease of superoxide dismutase (SOD) activity was exhibited, and catalase (CAT) was inhibited at the highest dose (40 mg/L). Reactive oxygen species (ROS) levels were significantly promoted at most exposure interval times except for the dose of 5 mg/L on day 21 in male and days 21 and 28 in female. Malonaldehyde (MDA) contents remarkable increased exposed to [C₆mim]Br. Besides, a notable increase was exhibited, which indicated an inducement of DNA damage with respect to control groups. Thus, we believed that [C₆mim]Br causes oxidative stress and DNA damage in zebrafish. Gender differences were insignificant in almost all the tested biomarkers, thus, male and female zebrafish could be mixed at a ratio of 1:1 in the future evaluation. The present study may also provide basic toxicology information for IL evaluation to aquatic organisms.