Simultaneous determination of rutin and ascorbic acid in a sequential injection lab-at-valve system

Area of Science:

• Analytical Chemistry
• Chemical Analysis
• Spectrophotometry

Background:

• Ascorbic acid (Asc) and rutin are vital antioxidants with health benefits.
• Accurate simultaneous determination of Asc and rutin is crucial for quality control and research.
• Existing methods may lack sensitivity, simplicity, or environmental friendliness.

Purpose of the Study:

• To develop a green, simple, accurate, and highly sensitive lab-at-valve procedure for simultaneous Asc and rutin determination.
• To utilize the pH-dependent reaction kinetics of 18-molybdo-2-phosphate Wells-Dawson heteropoly anion (18-MPA) with reducing agents.
• To enhance analytical performance through external reaction chambers and segmented reaction mixtures.

Main Methods:

• Sequential injection lab-at-valve procedure.
• Utilized 18-molybdo-2-phosphate Wells-Dawson heteropoly anion (18-MPA) and its reaction rate dependence on pH.
• Employed external reaction chamber and segmented reaction mixture for improved precision and sensitivity.
• Measured absorbance at the isobestic point for linear calibration graphs.

Main Results:

• Simultaneous determination of Asc and rutin achieved with high sensitivity and accuracy (1-2%).
• Linear calibration curves obtained in concentration ranges of 0.3-24 mgL⁻¹ for rutin and 0.2-14 mgL⁻¹ for Asc.
• Low detection limits achieved: 0.13 mgL⁻¹ for rutin and 0.09 mgL⁻¹ for Asc.
• Successful determination of Asc and rutin in ascorutin tablets.

Conclusions:

• The developed sequential injection method is effective for simultaneous Asc and rutin quantification.
• The method offers a green, simple, accurate, and sensitive alternative for analyzing these compounds.
• It demonstrates applicability in pharmaceutical analysis, specifically for ascorutin tablets.