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Filtration coefficient in cat hindlimb using protein concentration changes.

P D Watson¹, M B Wolf

¹Department of Physiology, University of South Carolina, School of Medicine, Columbia 29208.

The American Journal of Physiology

|January 1, 1989

Summary

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This study introduces a new method to measure capillary filtration coefficient (CFC) in cat skeletal muscle, distinguishing filtration from volume changes. The new method provides a more stable measurement of capillary filtration, resolving previous disputes.

Area of Science:

Physiology
Cardiovascular Research
Skeletal Muscle Physiology

Background:

The capillary filtration coefficient (CFC) in maximally vasodilated cat skeletal muscle has reported values that are disputed.
Previous methods using gravimetric and volumetric measurements may not accurately separate transcapillary filtration from vascular volume changes.

Purpose of the Study:

To develop and validate a novel method for measuring capillary filtration rates that is independent of vascular volume changes.
To resolve discrepancies in reported CFC values in cat skeletal muscle.

Main Methods:

Developed a new method measuring filtration rates via changes in venous protein concentration using Evan's blue-labeled albumin in an isolated cat hindlimb.
Calculated the perfusate filtration coefficient (PFFC) after a step increase in venous pressure, a method hypothesized to be unaffected by vascular volume fluctuations.

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Main Results:

The calculated PFFC was 0.0085 +/- 0.0015 ml.min-1.mmHg-1.100 g muscle-1 at perfusate flow rates > 15 ml.min-1.100 g muscle-1.
PFFC remained constant from 1 to 12 minutes post-venous pressure elevation.
In contrast, traditional CFC values decreased over the same time period.

Conclusions:

The novel PFFC measurement method provides a stable and reliable assessment of capillary filtration.
Observed differences between PFFC and CFC suggest that vascular volume changes confound traditional CFC measurements.
This study clarifies the measurement of capillary filtration in skeletal muscle, resolving previous discrepancies.