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Related Experiment Videos

Hypoxic reperfusion attenuates postischemic microvascular injury.

R J Korthuis1, J K Smith, D L Carden

  • 1Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport 71130-3932.

The American Journal of Physiology
|January 1, 1989
PubMed
Summary

Molecular oxygen is required for ischemia/reperfusion (I/R) injury in skeletal muscle. Preventing oxygen during reperfusion significantly reduces I/R injury, indicating oxygen

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Area of Science:

  • Physiology
  • Surgical Research

Background:

  • Reactive oxygen metabolites contribute significantly to skeletal muscle ischemia/reperfusion (I/R) injury.
  • Cytotoxic oxidants are presumed to be produced during reperfusion when molecular oxygen is reintroduced.

Purpose of the Study:

  • To test the hypothesis that molecular oxygen is essential for I/R injury in skeletal muscle.
  • To investigate the role of oxygen in reperfusion-induced vascular permeability and resistance changes.

Main Methods:

  • Isolated canine gracilis muscles underwent 4 hours of inflow occlusion.
  • Muscles were reperfused with either oxygenated or anoxic blood.
  • Vascular permeability (solvent drag reflection coefficient, sigma f) and total vascular resistance were measured.

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Main Results:

  • Reperfusion with oxygenated blood after ischemia significantly increased vascular permeability (decreased sigma f) and vascular resistance.
  • Reperfusion with anoxic blood resulted in less severe increases in vascular permeability and resistance compared to oxygenated reperfusion.
  • The solvent drag reflection coefficient decreased from 0.93 to 0.63 with oxygenated reperfusion, indicating increased permeability.

Conclusions:

  • Molecular oxygen is necessary for the development of significant ischemia/reperfusion injury in skeletal muscle.
  • Limiting oxygen availability during reperfusion can mitigate I/R-induced vascular damage.