Related Experiment Video
Updated: Oct 12, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Contribution of 3CD Region to the Virulence of Enterovirus 71
Jing Li1, Feng Gao2, Shu Bin Hao3
1Department of Virology, School of Public Health, Shandong University, Jinan 250012, Shandong, China; The Second Hospital of Shandong University, Jinan 250033, Shandong, China.
Abstract:
Enterovirus 71 is a neuroinvasive virus that is associated with severe neurological complications. We had earlier suggested that the replication capacity of a severe strain was higher than that of a mild strain. The recombinant 3CRV and 3CDRV virus strains were successfully rescued in our previous study. In the present study, we found no difference in virulence between 3CRV and severe strains. However, the capacity of replication and to cause cell injury of 3CDRV strain decreased in vitro, especially at 39.5 °C. Replacement of 3CD region in the severe strain led to milder symptoms, less body weight loss, and lower viral load in ICR mice. Histopathological findings indicated less severe injury in mice infected with 3CDRV strain. This study suggests that the 3CD region contributes to the attenuation of the severe strain, including its replication capacity and temperature sensitivity.
More Related Videos
10:52Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
Published on: October 22, 2013
12:20Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Related Concept Videos
Determinants of Bacterial Pathogenicity and Virulence
Regulation of Bacterial Virulence
Bacterial Gastroenteritis
Viral Meningitis
Respiratory Syncytial Virus Disease