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A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
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Bioprocessing Strategies for Pluripotent Stem Cells Based on Waddington's Epigenetic Landscape
Mee-Hae Kim1, Masahiro Kino-Oka1
1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Trends in Biotechnology
|November 11, 2017
Summary
Novel stem cell bioprocessing strategies leverage Waddington's epigenetic landscape to guide cellular decision-making. Biochemical engineering enhances these methods for improved cell-based therapies in research and clinical applications.
Area of Science:
- Biotechnology
- Developmental Biology
- Biochemical Engineering
Background:
- Advancing cell-based therapies necessitate robust cell culturing strategies.
- Understanding cellular decision-making is crucial for effective bioprocessing.
Purpose of the Study:
- To explore novel stem cell bioprocessing strategies.
- To apply Waddington's epigenetic landscape model to cell fate decisions.
- To enhance cell-based therapies through biochemical engineering.
Main Methods:
- Utilizing Waddington's epigenetic landscape as a conceptual framework.
- Analyzing cell behavior and its impact on cell signaling pathways.
- Investigating biochemical engineering approaches for bioprocess development.
Main Results:
- Conceptual framework for understanding stem cell differentiation pathways.
- Identification of key cell signaling events in early cell fate determination.
- Potential for enhanced bioprocesses through targeted engineering.
Conclusions:
- Waddington's epigenetic landscape offers a valuable model for stem cell bioprocessing.
- Biochemical engineering can optimize cell culturing for therapeutic applications.
- Novel strategies can improve the efficacy of cell-based therapies.
Keywords:
Waddington’s epigenetic landscapecell behaviorculture platformculture toolsnext-generation bioprocessingstem cell bioprocessingMore Related Videos
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