Related Experiment Video
Updated: Feb 19, 2026

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Published on: October 10, 2020
Glutathione Transferase U13 Functions in Pathogen-Triggered Glucosinolate Metabolism
Mariola Piślewska-Bednarek1, Ryohei Thomas Nakano2,3, Kei Hiruma2,4
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznań, Poland.
Glutathione S-Transferase U13 (GSTU13) is crucial for plant immunity, specifically linking glutathione to indole glucosinolate metabolism in Arabidopsis. This enzyme
Area of Science:
- Plant immunity
- Plant biochemistry
- Molecular plant pathology
Background:
- Glutathione (GSH) and indole glucosinolates (IGs) are vital for Arabidopsis immunity.
- The PENETRATION2 (PEN2) pathway metabolizes IGs for preinvasive resistance.
- GSH conjugation with IG metabolites was indirectly suggested.
Purpose of the Study:
- Identify components of the PEN2 pathway for IG metabolism.
- Investigate the role of Glutathione-S-Transferase class-tau member 13 (GSTU13) in plant immunity.
Main Methods:
- Genetic analysis of *gstu13* mutants.
- Metabolite profiling of PEN2 pathway end products.
- Pathogen susceptibility assays.
- Callose deposition assays.
Main Results:
- *gstu13* mutants are deficient in pathogen-triggered biosynthesis of PEN2 pathway end products.
- Lack of GSTU13 leads to increased susceptibility to fungal pathogens.
- *gstu13* seedlings fail to deposit callose upon flg22 epitope recognition.
- GSTU13 specifically mediates GSH's role in IG metabolism.
Conclusions:
- GSTU13 is essential for the PEN2 pathway's IG metabolism in Arabidopsis immunity.
- GSTU13 connects GSH to the PEN2 pathway, producing immune-active metabolites.
- This pathway contributes to innate immunity against fungal and bacterial pathogens.
More Related Videos
10:09A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC
Published on: March 15, 2017
08:31Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
Published on: March 29, 2019
Related Concept Videos
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Sulfur Assimilation
Phase II Reactions: Glucuronidation
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase
Drug Metabolism: Phase II Reactions
Bioactivation and Tissue Toxicity