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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: Feb 19, 2026

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

A Microdevice Platform Recapitulating Hypoxic Tumor Microenvironments.

Yuta Ando¹, Hoang P Ta¹, Daniel P Yen¹

¹Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, 90089, USA.

Scientific Reports

|November 11, 2017

Summary

This summary is machine-generated.

Researchers developed a novel microdevice to mimic tumor oxygen gradients, aiding cancer research. This platform facilitates understanding hypoxia

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Last Updated: Feb 19, 2026

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A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

Published on: September 20, 2016

Area of Science:

Oncology
Biomedical Engineering
Cancer Biology

Background:

Hypoxia, or low oxygen, is critical in tumor growth and treatment resistance.
Intratumoral oxygen gradients create diverse cellular environments within solid tumors.

Purpose of the Study:

To engineer a microdevice platform that accurately replicates intratumor oxygen gradients.
To provide a tool for studying hypoxia-driven cancer mechanisms and discovering new therapies.

Main Methods:

A microdevice was designed with a cell layer between diffusion barriers to establish oxygen gradients.
Numerical simulations and oxygen sensor measurements confirmed the gradient.
Spatially resolved hypoxic signaling was analyzed using immunostaining and gene expression assays.

Main Results:

The microdevice successfully generated and controlled oxygen gradients.
Hypoxia-targeted drug treatments showed spatially resolved effects on cancer cells.
The platform demonstrated compatibility with high-content imaging and high-throughput screening.

Conclusions:

The engineered microdevice platform effectively models tumor oxygen gradients.
This tool advances the study of hypoxia in cancer and aids therapeutic discovery.