Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conserved Binding Sites

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The TASK-1 and TASK-3 activator JG-C3-98 attenuates cold and mechanical responses in primary somatosensory neurons.

Frontiers in pharmacology·2026

Same author

Click. Screen. Degrade. A Miniaturized D2B Workflow for Rapid PROTAC Discovery.

Journal of medicinal chemistry·2026

Same author

Unveiling the ZNF384-INTS13-hnRNPC axis as a therapeutic vulnerability in cervical cancer.

Cell death & disease·2025

Same author

Molecular determinants of [Formula: see text] and cation transport in the human cation-dependent Cl<sup>-</sup>/[Formula: see text] exchanger AE4.

American journal of physiology. Cell physiology·2025

Same author

Rational design, synthesis, and evaluation of novel polypharmacological compounds targeting Na<sub>V</sub>1.5, K<sub>V</sub>1.5, and K<sub>2</sub>P channels for atrial fibrillation.

The Journal of biological chemistry·2025

Same author

Characterization by LC-MS/MS analysis of KLH vaccine conjugated with a tick antigen peptide.

The Analyst·2025

Same journal

The role of SUMOylation in regulating proteins that drive neuronal disease progression.

Biochemistry and biophysics reports·2026

Same journal

Plant-derived extracellular vesicles as bioactive nanoplatforms: Biogenesis, therapeutic evidence, and translational challenges.

Biochemistry and biophysics reports·2026

Same journal

Effects of metformin and exercise on Alzheimer's model induced by D-galactose in the rats: A behavioral, histological and BDNF gene study.

Biochemistry and biophysics reports·2026

Same journal

Comparative proteomics of human naso-oropharyngeal swabs from COVID19 patients for identification of potential biomarker candidates.

Biochemistry and biophysics reports·2026

Same journal

Salidroside and mesenchymal stem cells alleviate hypobaric hypoxia-induced lung injury and are associated with alterations in the gut-lung axis.

Biochemistry and biophysics reports·2026

Same journal

A colorimetric readout based on the PrestoBlue® dye as an objective method for measuring neutralizing antibodies against influenza virus in human serum samples.

Biochemistry and biophysics reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 19, 2026

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Predicting CK2 beta-dependent substrates using linear patterns.

Teresa Núñez de Villavicencio-Díaz¹, Yuliet Mazola¹, Yasser Perera Negrín²

¹Bioinformatics Group, Department of Systems Biology, Biomedical Research Area, Center for Genetic Engineering and Biotechnology, Playa, La Habana, Cuba.

Biochemistry and Biophysics Reports

|November 11, 2017

Summary

This summary is machine-generated.

This study identifies beta-dependent substrates of protein kinase CK2 (Casein Kinase 2), revealing their roles in viral infection, apoptosis, DNA repair, and RNA metabolism, primarily within the cell nucleus.

Keywords:

Beta-dependent substrates Bioinformatics CK2 Class-III substrates Holoenzyme-dependent phosphorylation Text mining

More Related Videos

Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Related Experiment Videos

Last Updated: Feb 19, 2026

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Area of Science:

Biochemistry and Molecular Biology
Cellular Signaling
Cancer Research

Background:

Protein kinase CK2 (Casein Kinase 2) is a critical enzyme involved in numerous cellular processes, often deregulated in diseases like cancer.
CK2's holoenzyme comprises catalytic (α/α´) and regulatory (β) subunits; the β subunit modulates kinase activity in a substrate-dependent manner.
Identifying CK2 substrates phosphorylated exclusively in the presence of the β subunit (beta-dependent substrates) is crucial for understanding CK2's cellular functions.

Purpose of the Study:

To develop a predictive method for identifying beta-dependent CK2 substrates.
To analyze the functional roles and cellular localization of these predicted beta-dependent substrates.
To advance the comprehensive characterization of CK2's regulatory mechanisms.

Main Methods:

Utilized motif-based prediction to design linear patterns for identifying beta-dependent CK2 substrates.
Applied high-throughput substrate prediction to experimentally determined CK2 substrates.
Integrated functional classification and network analysis to interpret the results.

Main Results:

Successfully developed predictive patterns for classifying beta-dependent CK2 substrates.
Identified potential roles for beta-dependent phosphorylation in viral infection, apoptosis, DNA repair, and RNA metabolism.
Found that human beta-dependent CK2 substrates are predominantly located in the nucleus, with some exhibiting nucleocytoplasmic shuttling.

Conclusions:

Beta-dependent phosphorylation by CK2 plays significant regulatory roles in key cellular processes and viral infection.
The predictive framework enables broader identification of beta-dependent CK2 substrates.
Understanding the localization and function of these substrates provides insights into CK2's role in health and disease.