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Targeted Gene Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases

Sylvia Merkert1,2,3, Ulrich Martin4,5,6

  • 1Department of Cardiothoracic, Transplantation and Vascular Surgery, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover, Germany.

Advances in Biochemical Engineering/Biotechnology
|November 11, 2017
PubMed
Summary

Induced pluripotent stem cell (iPSC) technology and gene editing tools like CRISPR enable precise genome manipulation in human stem cells. This review covers current applications and future challenges for regenerative medicine and research.

Keywords:
CRISPR/Cas9Homologous recombinationNHEJTALENZFN

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