Related Experiment Video

Updated: Feb 19, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K

Mechanisms of transcriptional regulation by p53

Kelly D Sullivan1,2, Matthew D Galbraith1,2, Zdenek Andrysik1,2

  • 1Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.

Cell Death and Differentiation
|November 11, 2017
PubMed

Insights

The tumor suppressor p53 (also known as TP53) is crucial for controlling cell growth. Recent research clarifies how p53 regulates genes, offering new insights into cancer prevention and treatment strategies.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • The p53 protein (TP53) is a critical tumor suppressor involved in regulating numerous target genes.
  • p53 activity is frequently lost in cancers due to mutations or repression by factors like MDM2.
  • Despite extensive research, the complete p53 signaling pathway is not fully understood.

Purpose of the Study:

  • To review recent advancements in understanding p53-dependent transcriptional control mechanisms.
  • To highlight key areas of p53 function in gene regulation and tumor suppression.

Main Methods:

  • This review synthesizes current research on p53 transcriptional regulation.
  • Focuses on five key aspects of p53's function in gene expression.

Main Results:

  • Explores the distinct roles of p53's N-terminal transactivation domains.
  • Examines the regulatory functions of p53's C-terminal domain.
  • Presents evidence for p53 acting as a direct transcriptional activator, not a repressor.
  • Discusses p53's ability to bind enhancers in various chromatin contexts.
  • Investigates context-dependent modifications of the p53 transcriptional program.

Conclusions:

  • Recent findings deepen our understanding of p53's complex transcriptional control.
  • These insights are vital for developing targeted cancer therapies by reactivating p53 function.

More Related Videos

Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K
Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

246

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.2K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.2K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.6K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K