Related Experiment Video
Updated: Aug 16, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
Genotoxic effects of intragastrically administered benzo[a]pyrene in rat liver and intestinal cells
E Mullaart1, M Buytenhek, A Brouwer
1TNO Institute for Experimental Gerontology, Rijswijk, The Netherlands.
Abstract:
The genotoxic effects of intragastrically administered benzo[a]pyrene (BaP) were studied in isolated rat liver and intestinal cells. We found that BaP was unable to induce unscheduled DNA synthesis in rat parenchymal liver cells in vivo. In contrast, alkaline elution showed that at 5 h after administration of BaP a considerable number of alkali-labile sites was present in the DNA of both intestinal cells and parenchymal liver cells, but not in that of non-parenchymal liver cells. The possibility is discussed that these sites were induced by radical intermediates, generated during the metabolization of BaP.
Related Concept Videos
Physical Properties of Amines
Mutagenicity and Carcinogenicity
Bioactivation and Tissue Toxicity
Toxicity Testing in Animals

