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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
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In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Southern Blot

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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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Related Experiment Video

Updated: Feb 19, 2026

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Published on: June 1, 2018

Catching RNAs on chromatin using hybridization capture methods.

Martin Machyna, Matthew D Simon

Briefings in Functional Genomics

|November 11, 2017

Summary

This summary is machine-generated.

Long noncoding RNAs (lncRNAs) interact with chromatin. Hybridization capture methods like CHART, ChIRP, and RAP detect these interactions genome-wide, aiding in understanding lncRNA biological roles.

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Chromatin Isolation by RNA Purification ChIRP

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

Long noncoding RNAs (lncRNAs) are increasingly recognized for their crucial biological functions.
Evidence suggests that some lncRNAs associate with chromatin, influencing gene regulation.
Understanding lncRNA-chromatin interactions is key to deciphering their roles.

Purpose of the Study:

To review the principles of hybridization capture methods for detecting lncRNA interactions.
To focus on three prominent protocols: CHART, ChIRP, and RAP.
To aid researchers in applying and interpreting these techniques.

Main Methods:

Hybridization capture methods utilize antisense oligonucleotides for RNA enrichment.
These techniques involve RNA purification from cross-linked chromatin extracts.
Genome-wide detection of lncRNA interaction sites at high resolution is achieved.

Main Results:

These methods provide insights into lncRNA localization within the genome.
They reveal interactions between lncRNAs and proteins.
The review highlights commonalities and differences among CHART, ChIRP, and RAP protocols.

Conclusions:

Hybridization capture techniques are powerful tools for studying lncRNA functions.
Understanding the nuances of different protocols (CHART, ChIRP, RAP) is essential for accurate results.
These methods advance our comprehension of lncRNA-mediated biological processes.