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Updated: Feb 19, 2026

Lensless Fluorescent Microscopy on a Chip
Published on: August 17, 2011
Lensless Photoluminescence Hyperspectral Camera Employing Random Speckle Patterns
Karel Žídek1, Ondřej Denk2, Jiří Hlubuček2
1Regional Centre for Special Optics and Optoelectronic Systems (TOPTEC), Institute of Plasma Physics, Academy of Sciences of the Czech Republic, Za Slovankou 1782/3, 182 00, Prague 8, Czech Republic. zidek@ipp.cas.cz.
We developed a simple spectrally-resolved photoluminescence imaging system using a single-pixel camera and laser speckle patterns. This technique reconstructs hyperspectral images with high efficiency and broad applicability for various excitation sources.
Area of Science:
- Optics and Photonics
- Spectroscopy
- Image Reconstruction
Background:
- Hyperspectral imaging traditionally requires complex setups.
- Compressive sensing offers novel imaging approaches.
- Photoluminescence imaging provides valuable material insights.
Purpose of the Study:
- To demonstrate a simplified spectrally-resolved photoluminescence imaging setup.
- To leverage single-pixel camera techniques for hyperspectral imaging.
- To explore laser speckle patterns for image encoding.
Main Methods:
- Utilized a single-pixel camera architecture with a spectrometer as the detector.
- Employed random laser speckle patterns for image encoding.
- Reconstructed hyperspectral images from collected data.
Main Results:
- Successfully generated spectrally-resolved photoluminescence images of model scenes.
- Demonstrated the simplicity and effectiveness of the proposed setup.
- Analyzed the impact of speckle pattern parameters on imaging quality.
Conclusions:
- The developed technique offers a simple and versatile method for spectrally-resolved photoluminescence imaging.
- This approach is compatible with various coherent excitation sources and spectral detection ranges.
- It presents a promising alternative to traditional hyperspectral imaging methods.
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