Significant increase in cyanide degradation by Bacillus sp. M01 PTCC 1908 with response surface methodology...

Area of Science:

• Environmental microbiology
• Biotechnology
• Industrial microbiology

Background:

• Cyanide is a highly toxic industrial chemical.
• Biodegradation offers an affordable and eco-friendly alternative for cyanide remediation.
• Effective microbial solutions are needed for cyanide-contaminated sites.

Purpose of the Study:

• To isolate and identify cyanide-degrading bacteria from contaminated mine sites.
• To optimize culture conditions for enhanced cyanide biodegradation by the selected bacterial strain.
• To evaluate the efficiency and potential of the optimized biodegradation process.

Main Methods:

• Isolation and screening of bacteria from cyanide-contaminated Muteh gold mine.
• Identification of Bacillus sp. M01 with high cyanide tolerance (15 mM).
• Optimization of culture conditions using Plackett-Burman design and response surface methodology.
• Quantification of cyanide degradation using ANOVA and t-tests.

Main Results:

• Bacillus sp. M01 (PTCC No.: 1908) demonstrated significant cyanide tolerance and degradation capabilities.
• Optimized conditions led to a 2.35-fold increase in degradation efficiency compared to pre-optimization.
• High coefficient of determination (R² = 91%) indicates the robustness of the optimization model.
• The optimized process achieved 86% degradation of 10 mM cyanide within 48 hours.

Conclusions:

• Bacillus sp. M01 is a promising candidate for efficient cyanide biodegradation.
• Optimized microbial processes offer a sustainable solution for cyanide waste management.
• The study provides a patented, effective method for industrial cyanide remediation.