Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

9.0K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
9.0K
X-ray Imaging01:24

X-ray Imaging

10.7K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.3K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
X-ray Crystallography02:18

X-ray Crystallography

26.4K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ABSCISIC ACID-INSENSITIVE 5 (ABI5) Orchestrates Seasonal Growth Cessation and Wood Formation Inhibition in <i>Populus tomentosa</i>.

Plants (Basel, Switzerland)·2026
Same author

Long-term clinical outcomes of minimally invasive transforaminal lumbar interbody fusion (Mis-TLIF) compared with open TLIF(O-TLIF): A retrospective cohort study for at least 5 years.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Macroscopic and microscopic investigation of adsorption mechanisms of phenanthrene and its derivatives on polyacrylonitrile microplastics.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Hierarchical Nanoarchitectonics of Hyaluronidase-Powered and Chitosan-Interfaced Liposomal Microneedles for Chemotherapeutic Extravasation Treatment.

ACS applied materials & interfaces·2026
Same author

Anchoring Triple Polar Sites in COFs to Tailor Electronic and Surface Properties for High Optoelectronic Performance.

Nano letters·2026
Same author

Lung function as a mediator: bridging appendicular skeletal muscle mass and dementia in middle-aged and older Chinese adults.

European journal of medical research·2026

Related Experiment Video

Updated: Feb 19, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.7K

X-ray luminescence computed tomography using a focused x-ray beam.

Wei Zhang1, Michael C Lun1, Alex Anh-Tu Nguyen1

  • 1University of California, Merced, School of Engineering, Merced, California, United States.

Journal of Biomedical Optics
|November 12, 2017
PubMed
Summary

This study introduces a novel focused X-ray luminescence computed tomography (XLCT) system using a single optical fiber bundle and photomultiplier tube, significantly reducing measurement times. This advancement enables faster and more sensitive XLCT imaging for various applications.

Keywords:
imagingopticsx-rayx-ray luminescence computed tomography

More Related Videos

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
10:42

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

Published on: June 16, 2016

9.8K
Author Spotlight: Advancements in X-ray CT Tool Chain for Tree Core Analysis
06:56

Author Spotlight: Advancements in X-ray CT Tool Chain for Tree Core Analysis

Published on: September 22, 2023

1.7K

Related Experiment Videos

Last Updated: Feb 19, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.7K
In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
10:42

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

Published on: June 16, 2016

9.8K
Author Spotlight: Advancements in X-ray CT Tool Chain for Tree Core Analysis
06:56

Author Spotlight: Advancements in X-ray CT Tool Chain for Tree Core Analysis

Published on: September 22, 2023

1.7K

Area of Science:

  • Medical Imaging
  • Biomedical Engineering
  • Physics

Background:

  • Collimator-based narrow beam X-ray luminescence computed tomography (XLCT) systems suffer from low efficiency and sensitivity, leading to long measurement times.
  • Existing XLCT methods often require extensive data acquisition, limiting their practical application.

Purpose of the Study:

  • To develop a faster and more sensitive XLCT imaging system.
  • To overcome the limitations of traditional collimator-based XLCT.
  • To demonstrate the feasibility of a focused X-ray beam approach for XLCT.

Main Methods:

  • Developed a novel XLCT system utilizing a focused X-ray beam generated by an X-ray tube with a polycapillary lens.
  • Employed a single optical fiber bundle and a photomultiplier tube (PMT) for photon detection.
  • Performed numerical simulations and phantom experiments to validate the system's performance.

Main Results:

  • Achieved a 1200-fold increase in X-ray photon density compared to collimated beams.
  • Reduced total measurement time to 12.5 minutes.
  • Successfully reconstructed multiple targets in simulations and two closely spaced targets (0.4 mm edge-to-edge) in phantom experiments.

Conclusions:

  • The focused X-ray beam XLCT system significantly enhances imaging speed and sensitivity.
  • The use of a single optical fiber bundle and PMT is effective for XLCT data acquisition.
  • This approach represents a substantial improvement for XLCT imaging, enabling more efficient preclinical and clinical applications.