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Updated: Feb 19, 2026
Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
Relationship of particulate matter and ozone with 3-nitrotyrosine in the atmosphere
Tatsuo Ito1, Keiki Ogino1, Kenjiro Nagaoka1
1Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, 2-5-1, Shikata-cho, Kita-ku, Okayama, Japan.
Abstract:
The prevalence of allergic diseases has increased in the past few decades. Bio-aerosol proteins and their chemical modifications, such as 3-nitrotyrosine (3-NT), in the atmosphere have been attracting attention due to their promotive effects on allergies. 3-NT is generated from the amino acid, tyrosine, through a reaction with ozone (O3) and nitrogen dioxide (NO2). However, the underlying mechanisms have not yet been elucidated in detail. Therefore, we measured 3-NT and evaluated the relationships among 3-NT and various pollutants such as sulfur dioxide (SO2), NOx (NO + NO2), ozone (O3), PM7, total suspended particulate matter (TSP) containing proteins, humidity, and temperature. 3-NT positively correlated with O3, SO2, humidity, and temperature, and negatively correlated with NOx. A multiple regression analysis showed that 3-NT positively associated with O3, humidity, and PM7. O3 positively associated with 3-NT and PM7, and negatively associated with NOx and humidity. These results suggest that 3-NT is generated from PM proteins through a reaction with O3 under high humidity conditions, and that the measurement of 3-NT is important and useful for the research of O3.
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