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P85 regulates neuronal migration through affecting neuronal morphology during mouse corticogenesis.
Xinran Cheng1, Kaikai Li1, MengMeng Liu1
1College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Cell and Tissue Research
|November 14, 2017
Summary
The P85 subunit is crucial for guiding migrating neurons in the developing brain. Overexpressing P85 disrupts neuronal migration and alters cell shape, impacting corticogenesis.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Mammalian cortical development involves projection neuron migration guided by radial glial cells.
- The PI3K-Akt-mTOR signaling pathway is vital for brain development.
- The specific role of the P85 subunit in cortical development remains largely unknown.
Purpose of the Study:
- To investigate the function of the P85 subunit in mammalian cortical development.
- To determine the impact of P85 on neuronal migration and morphology.
Main Methods:
- Utilized in utero electroporation in mouse models.
- Overexpressed the P85 subunit in developing cortical neurons.
- Analyzed neuronal migration, leading process length and branching, and neuronal fate markers.
Main Results:
- Overexpression of P85 significantly impaired cortical neuronal migration.
- P85 overexpression led to shorter leading processes with increased branching in migrating neurons.
- The ultimate fate of newborn cortical neurons was not affected by P85 overexpression.
Conclusions:
- The P85 subunit plays an essential role in regulating neuronal migration during mouse corticogenesis.
- P85 influences neuronal morphology, specifically affecting the leading process during migration.
- These findings highlight P85 as a key factor in the complex process of cortical development.
