A Transgenic Core Facility's Experience in Genome Editing Revolution.

Area of Science:

• Biomedical Research
• Genetics
• Animal Models

Background:

• Animal models, especially rodents, are crucial for biomedical research.
• Transgenic core facilities are essential for producing and managing these models.
• Traditional methods for animal model production are often time-consuming.

Purpose of the Study:

• To describe the impact of CRISPR/Cas9 on animal model production.
• To share practical guidelines and lessons learned from implementing CRISPR/Cas9 technology.
• To provide insights into future directions in animal model generation.

Main Methods:

• Adoption of CRISPR/Cas9 technology in 2014 by a transgenic core facility.
• Performed over 200 genome editing projects using CRISPR/Cas9.
• Shift from embryonic stem cell-based methods to direct genomic editing in rodent embryos.

Main Results:

• CRISPR/Cas9 significantly simplified and improved the efficiency of animal model production.
• Direct genomic editing in rodent embryos proved more effective than conventional methods.
• Extensive experience gained from over 200 genome editing projects.

Conclusions:

• CRISPR/Cas9 technology has transformed animal model generation.
• Practical guidelines are provided for efficient use of this technology.
• Future advancements in genome editing will continue to impact biomedical research.