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Updated: Feb 19, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
Published on: February 2, 2024
Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq
1Oregon State University, 1500 SW Chandler Ave, Bend, OR, 97702, USA. majerczc@oregonstate.edu.
Abstract:
RNA sequencing (RNAseq) enables transcriptional profiling of many organisms. This chapter describes the use of RNAseq in prokaryotes to identify quorum sensing (QS)-controlled transcripts by comparing samples from QS-induced and -uninduced conditions. Briefly, each RNA sample is converted to ds-cDNA in a method that limits amplification of ribosomal RNA species. The ds-cDNA contains adapters that enable sequencing and quantification by next-generation sequencing (NGS).
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