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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Feb 19, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

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Identifying the m⁶A Methylome by Affinity Purification and Sequencing.

Phillip J Hsu^1,2,3, Chuan He^4,5,6

¹Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, 929 E. 57th Street, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 14, 2017

Summary

This summary is machine-generated.

N-6-methyladenosine (m6A) is a crucial mRNA regulator. This study introduces m6A-seq, a high-throughput sequencing method to map m6A sites across the transcriptome.

Keywords:

Affinity purification Methylome N 6-methyladenosine Sequencing Transcriptome

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Published on: April 28, 2022

Area of Science:

Molecular Biology
Epigenetics
RNA Biology

Background:

N-6-methyladenosine (m6A) is the most prevalent internal modification in eukaryotic messenger RNA (mRNA).
m6A is increasingly recognized as a critical regulator of posttranscriptional mRNA processes.
Understanding the distribution and function of m6A sites is essential for deciphering gene regulation.

Purpose of the Study:

To present a detailed protocol for m6A-seq.
To enable large-scale mapping of m6A sites within the transcriptome.
To facilitate the study of the m6A methylome.

Main Methods:

High-throughput sequencing (m6A-seq) was employed.
Affinity purification techniques were utilized to isolate m6A-modified RNA.
The protocol details the steps for transcriptome-wide identification of m6A sites.

Main Results:

The study successfully outlines a protocol for m6A-seq.
This method allows for the mapping of m6A sites on a transcriptome-wide scale.
The protocol facilitates the characterization of the m6A methylome.

Conclusions:

m6A-seq provides a powerful tool for investigating m6A modifications.
The presented protocol enables comprehensive analysis of the m6A methylome.
This methodology will advance the understanding of m6A's role in mRNA regulation.