Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Instrument-Software Synergy in Proteomics: Systematic Evaluation across Mass Spectrometry Platforms, Search Engines, and Rescoring Methods.

Journal of proteome research·2026
Same author

Community-driven advances in computational mass spectrometry: The perspective of EuBIC-MS members.

Journal of proteomics·2026
Same author

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
Same author

bio.tools: an expanded web service for research software in the life sciences.

Nucleic acids research·2026
Same author

Synthesis of Cyclopropene-Modified Fatty Acids Allows Single-Cell Quantification of Uptake by Immune Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Comprehensive Lipidomic Profiling Reveals Distinct Metabolic Remodeling during Differentiation of Human Monocyte-Derived Macrophages.

Journal of proteome research·2026

Related Experiment Video

Updated: Feb 18, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

13.4K

Human Dendritic Cells with Th2-Polarizing Capacity: Analysis Using Label-Free Quantitative Proteomics.

Leonie Hussaarts1, Maria Mardalena Martini Kaisar, Arzu Tugce Guler

  • 1Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.

International Archives of Allergy and Immunology
|November 14, 2017
PubMed
Summary

Helminth antigens alter dendritic cell (DC) proteomes, influencing T helper cell polarization. This study reveals how DC metabolism and stress proteins contribute to Th2 immune responses.

Keywords:
Dendritic cellsHelminthsOmega-1ProteomeT helper 2 responses

More Related Videos

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

15.3K
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
10:03

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Published on: December 12, 2014

8.5K

Related Experiment Videos

Last Updated: Feb 18, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

13.4K
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

15.3K
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
10:03

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Published on: December 12, 2014

8.5K

Area of Science:

  • Immunology
  • Proteomics
  • Cellular Biology

Background:

  • Dendritic cells (DCs) are key immune sentinels that mature and migrate to lymph nodes to initiate T cell responses.
  • While helminths are known to induce T helper 2 (Th2) cell polarization via DCs, the precise molecular mechanisms are not fully understood.

Purpose of the Study:

  • To perform a proteomic analysis of helminth antigen-stimulated DCs.
  • To elucidate cellular processes underlying DC-mediated immune polarization.

Main Methods:

  • Monocyte-derived DCs from 9 donors were analyzed for maturation and polarization.
  • Stimulation involved Th1- and Th2-polarizing pathogen-derived molecules.
  • Proteomic analysis was conducted using liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry.

Main Results:

  • Lipopolysaccharide (LPS) induced proteins related to metabolic, cellular, and immune processes.
  • Interferon-gamma (IFN-γ) conditioned Th1-polarizing DCs showed accelerated maturation with altered cytoskeletal and immune regulatory proteins.
  • Schistosoma mansoni antigens (soluble egg antigens and omega-1) increased specific ribosomal and transferase proteins while decreasing antigen processing proteins.

Conclusions:

  • DC proteins involved in immunological synapse interactions, cellular metabolism, and stress are implicated in promoting Th2 polarization.
  • Understanding these proteomic changes offers insights into DC function in helminth infections.