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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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Next-Generation Sequencing in Diagnostic Pathology.

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    Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
    |November 14, 2017
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    Summary
    This summary is machine-generated.

    Next-generation sequencing (NGS) provides detailed molecular data from tissue samples. Pathologists must understand NGS utility and limitations for accurate patient management by integrating molecular and morphological findings.

    Keywords:
    Diagnostic pathologyNext-generation sequencing

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    Area of Science:

    • Pathology
    • Genomics
    • Biotechnology

    Background:

    • Tissue interrogation is crucial for patient diagnosis and management.
    • Pathologists traditionally rely on morphology and limited biomarker analysis.
    • Advances in sequencing technology enable detailed molecular analysis of tissue.

    Purpose of the Study:

    • To describe the principles of next-generation sequencing (NGS) technologies.
    • To discuss the challenges and limitations in interpreting NGS data.
    • To provide a framework for classifying NGS-generated data for pathologists.

    Main Methods:

    • Review of next-generation sequencing (NGS) principles.
    • Discussion of data interpretation caveats in molecular pathology.
    • Presentation of a data classification scheme for NGS findings.

    Main Results:

    • NGS technologies generate vast amounts of molecular data from tissue.
    • Effective patient management requires integration of molecular and morphological data.
    • Understanding NGS utility and limitations is essential for pathologists.

    Conclusions:

    • Pathologists need to comprehend NGS principles and data interpretation.
    • A structured approach to classifying NGS data aids integration with morphology.
    • Familiarity with NGS terminology is vital for leveraging molecular insights in patient care.