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When Do Two-Stage Processes Outperform One-Stage Processes?

Steffen Klamt1, Radhakrishnan Mahadevan2, Oliver Hädicke1

  • 1Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstraße 1, 39106 Magdeburg, Germany.

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|November 14, 2017
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Summary
This summary is machine-generated.

Two-stage fermentation (TSF) does not always outperform one-stage fermentation (OSF). Careful assessment of substrate uptake, yield, and productivity trade-offs is crucial for optimizing biotechnological processes.

Keywords:
Escherichia colidynamic metabolic controldynamic process strategiesmetabolic engineeringtwo-stage fermentation

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Metabolic Engineering

Background:

  • Volumetric productivity is critical in biotechnological processes, alongside product yield and titer.
  • A trade-off exists between biomass production and target product formation, preventing simultaneous optimization of yield and volumetric productivity.
  • Two-stage fermentations (TSFs) with separated growth and production phases are gaining interest for improving bioprocess productivity and yield.

Purpose of the Study:

  • To systematically analyze whether and under which conditions TSF guarantees superior productivity compared to one-stage fermentation (OSF).
  • To identify challenges and potential solutions for designing competitive TSF processes.
  • To evaluate the influence of various factors on the relative performance of OSF and TSF.

Main Methods:

  • Mathematical modeling was employed to compare TSF and OSF performance.
  • Analysis focused on the impact of specific substrate uptake rates during production phases.
  • Investigated strategies like enforced ATP wasting to enhance substrate utilization in TSF.

Main Results:

  • TSF does not automatically ensure higher volumetric productivity than OSF.
  • A significant decrease in specific substrate uptake rate during production phases challenges TSF competitiveness.
  • OSF may be more suitable when high product yield is a primary economic constraint.

Conclusions:

  • A thorough evaluation of trade-offs between substrate uptake rates, yields, and productivity is essential when choosing between OSF and TSF.
  • Strategies to improve substrate utilization in the production phase are key for TSF superiority.
  • The choice between OSF and TSF depends on specific process constraints and optimization goals.