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Updated: Feb 18, 2026
Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics
Published on: January 22, 2020
Preparation of a beta-tricalcium phosphate nanocoating and its protein adsorption behaviour by quartz crystal
Dongwen Pang1, Liyong He1, Luxi Wei1
1Guangdong Key Laboratory of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China.
Abstract:
Beta-tricalcium phosphate (β-TCP) nanocoatings, which can be analysed using a quartz crystal microbalance with dissipation technique (QCM-D), were fabricated on a gold surface by electrophoretic deposition. The influences of electric field intensity and electrophoresis time were investigated. The adsorption behaviours of bovine serum albumin (BSA) and lysozyme (LSZ) on Au and β-TCP surfaces were observed in real time by QCM-D. The homogeneous β-TCP nanocoating with moderately sized particles on gold surface was fabricated at 25V/cm for 5min, and it met the requirements for the QCM-D experiment. The adsorption behaviour of BSA was different from that of LSZ, which was caused by the differences of protein properties. The adsorption quantity of BSA on a β-TCP surface was higher than that on a gold surface. However, the adsorption amount of LSZ on a β-TCP surface was lower than that on a gold surface. The electrostatic force was the major factor affecting the adsorption quantities of BSA and LSZ on Au and β-TCP surfaces based on the investigation of various factors. The findings reported here will be useful for understanding the mechanism of the interaction between biomaterials and proteins.
