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Adventitious viruses in insect cell lines used for recombinant protein expression
Christoph Geisler1, Donald L Jarvis2
1GlycoBac LLC, 1938 Harney Street, Laramie, WY 82072, USA.
Protein Expression and Purification
|November 15, 2017
Summary
Adventitious viruses contaminate insect cell lines used for protein expression and biomanufacturing, impacting research and product safety. New virus-free insect cell lines offer safer alternatives for research and industry.
Area of Science:
- Biotechnology and Molecular Biology
- Cell Line Development and Characterization
- Virology and Infectious Diseases
Background:
- Insect cell lines are crucial for recombinant protein expression, utilized in both research and the production of biopharmaceuticals.
- Recent discoveries revealed persistent adventitious virus infections in commonly used insect cell lines.
- These viral contaminations pose risks to research integrity and the safety of manufactured biological products.
Purpose of the Study:
- To review the scientific and patent literature concerning adventitious viruses in insect cell lines.
- To identify affected insect cell lines and discuss the implications of viral contamination.
- To highlight the development and availability of new, virus-free insect cell lines.
Main Methods:
- Comprehensive literature review of scientific publications and patents.
- Analysis of data on adventitious virus detection in insect cell lines.
- Identification and characterization of newly developed virus-free insect cell lines.
Main Results:
- Confirmation of persistent adventitious virus infections in several widely used insect cell lines.
- Identification of specific insect cell lines affected by these viral contaminations.
- Isolation and characterization of novel insect cell lines free from adventitious viruses.
Conclusions:
- Adventitious virus contamination in insect cell lines presents significant challenges for research reproducibility and biopharmaceutical safety.
- The development of virus-free insect cell lines is critical for ensuring reliable research outcomes and safe biological manufacturing.
- These new cell lines represent improved tools for scientific investigation and safer platforms for biopharmaceutical production.
Keywords:
Adventitious virusesBaculovirus insect cell systemInsect cell linesSf-rhabdovirusSpodoptera frugiperda cells
