Region and cell-type resolved quantitative proteomic map of the human heart

Area of Science:

• Cardiovascular Biology
• Proteomics
• Systems Biology

Background:

• Heart diseases are a leading global cause of mortality.
• Comprehensive understanding of the human heart's protein composition is limited.
• Existing proteomic data for the heart is insufficient for detailed analysis.

Purpose of the Study:

• To create a comprehensive proteome map of the healthy human heart.
• To quantify protein identities and abundances in various anatomical regions and cell types.
• To model the heart proteome at the subcellular level.

Main Methods:

• High-resolution mass spectrometry-based proteomics was employed.
• 16 anatomical regions and 3 major cardiac cell types were analyzed.
• Protein copy numbers per cell and organellar assignments were integrated.

Main Results:

• Over 10,700 proteins were quantified from low microgram sample amounts.
• A subcellular model of the heart proteome was constructed.
• Cardiac fibroblasts revealed potential cell surface markers.
• Analysis of atrial fibrillation showed distinct mitochondrial dysfunctions.

Conclusions:

• The human heart proteome map provides a valuable resource for cardiovascular research.
• The map facilitates the study of normal heart function and disease mechanisms.
• Identified markers and dysfunctions offer insights into cardiac pathologies like atrial fibrillation.