Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

5.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.7K
RNA Structure01:19

RNA Structure

7.8K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.8K
RNA Structure01:23

RNA Structure

79.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.3K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil extracellular trap (NET)-related index as an indicator of periprosthetic joint infection.

Journal of bone and joint infection·2026
Same author

Electrochemical Oxidation of Water Through Conducting Polymer for Hydroxyl Radical Generation at Ultra-Low Voltage.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Genomic mapping reveals cisplatin disruption of protein phosphorylation signalling genome-wide.

Metallomics : integrated biometal science·2026
Same author

Multi-Dimensional Insights Into the Surface and Interfaces of Battery Materials by Time-of-Flight Secondary Ion Mass Spectrometry.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

In-Situ Metabolic Profiling of Single Living Cells by Liquid Secondary Ion Mass Spectrometry.

Analytical chemistry·2026
Same author

Selection and Optimization of a High-Affinity FGFR4 Aptamer that Blocks FGF19-FGFR4 Signaling.

Analytical chemistry·2026
Same journal

Peripheral B-cell receptor repertoire predicts immune-related adverse events following immune checkpoint inhibitor therapy in advanced renal cell carcinoma.

Scientific reports·2026
Same journal

Effects of black soldier fly (Hermetia illucens L.) larvae zoocompost on the mineral element content of blue honeysuckle berries.

Scientific reports·2026
Same journal

Investigation on absorption refrigeration performance of R1243zf with imidazolium ionic liquid as the working pairs.

Scientific reports·2026
Same journal

DeepTriage-CN: integrating clinical text with vital signs for emergency department admission prediction in an aging population.

Scientific reports·2026
Same journal

Gold nanoparticles as dual-action antiviral agents: disruption of SARS-CoV-2 viral envelopes and RNA integrity.

Scientific reports·2026
Same journal

Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Feb 18, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.9K

Triplex-quadruplex structural scaffold: a new binding structure of aptamer.

Tao Bing1,2, Wei Zheng1, Xin Zhang1,2

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Scientific Reports
|November 15, 2017
PubMed
Summary
This summary is machine-generated.

A novel aptamer forms a unique triplex-quadruplex structure upon binding codeine. This structure inhibits polymerase activity, suggesting potential applications in gene regulation.

More Related Videos

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.0K
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.2K

Related Experiment Videos

Last Updated: Feb 18, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.9K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.0K
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Nucleic acids form non-canonical structures like G-quadruplexes and triplexes, enabling functions beyond genetic coding.
  • Aptamers are functional nucleic acids that achieve target specificity through unique tertiary structures.
  • Few aptamer structures are characterized despite numerous reported aptamers.

Purpose of the Study:

  • To report a novel triplex-quadruplex hybrid scaffold formed by a codeine-binding aptamer (CBA).
  • To investigate the structural changes induced by codeine binding to the CBA.
  • To explore the potential applications of this aptamer structure in gene regulation.

Main Methods:

  • Characterization of the G-rich DNA aptamer sequence and its derivatives.
  • Analysis of the aptamer's tertiary structure formation upon codeine binding using biophysical techniques (implied).
  • Investigation of codeine's binding site within the triplex-quadruplex hybrid structure.
  • Assessment of the effect of codeine-induced structure on polymerase activity.

Main Results:

  • A novel triplex-quadruplex hybrid scaffold is formed by the codeine-binding aptamer (CBA).
  • Codeine binding induces the formation of a complex structure comprising a G-quadruplex and a G·GC triplex, with codeine at the junction.
  • Codeine binds to the split CBA moieties by inducing the formation of the hybrid scaffold, not to individual moieties.
  • The codeine-induced structure formation inhibits polymerase activity.

Conclusions:

  • The codeine-binding aptamer (CBA) forms a unique triplex-quadruplex hybrid structure upon codeine interaction.
  • This structural transformation, mediated by codeine, has functional implications, including polymerase inhibition.
  • The CBA and its induced structure present potential for applications in gene regulation technologies.