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Related Concept Videos

Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
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Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
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Related Experiment Video

Updated: Feb 18, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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O Cdc7 kinase where art thou?

Robert A Sclafani1, Jay R Hesselberth2

  • 1University of Colorado School of Medicine, Aurora, CO, USA. robert.sclafani@ucdenver.edu.

Current Genetics
|November 15, 2017
PubMed
Summary

Cdc7 protein kinase, crucial for DNA replication and cancer therapy, has been localized to origins of replication using a novel genomic method. This finding confirms its role in DNA replication and other genomic processes.

Keywords:
Calling cardsCdc7ChromatinDDKDNA replicationOriginsYeast

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cdc7 protein kinase (DDK) is essential for DNA replication initiation in eukaryotes.
  • DDK is a validated target for cancer therapy, with inhibitors currently in clinical trials.
  • Despite its known function, Cdc7's precise cellular localization, particularly at replication origins, remained elusive.

Purpose of the Study:

  • To localize Cdc7 protein kinase within the cell and at specific genomic regions.
  • To investigate the presence of Cdc7 at functional origins of DNA replication.
  • To validate a novel genomic method for protein localization.

Main Methods:

  • Utilized the "Calling Card" system, a retrotransposon-based method for genome-wide DNA-binding protein localization.
  • Employed deep-sequencing to analyze the distribution of Cdc7 across the genome.
  • Correlated identified Cdc7 binding sites with known functional origins of replication.

Main Results:

  • Cdc7 was localized to numerous genomic regions.
  • Cdc7 showed enrichment at functional origins of DNA replication.
  • The Calling Card system proved effective for mapping protein localization.

Conclusions:

  • Cdc7 kinase is broadly distributed throughout the genome but is specifically enriched at active replication origins.
  • These findings support DDK's involvement in DNA replication, mutagenesis, chromatid cohesion, and meiotic recombination.
  • The Calling Card system is a valuable tool for identifying functional origins in various eukaryotic systems.