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High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
Published on: December 28, 2015
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Animal Cell Expression Systems.
1National Institute of Bioprocessing Research & Training (NIBRT), Dublin 4, Ireland.
Advances in Biochemical Engineering/Biotechnology
|November 15, 2017
Summary
Controlling protein glycosylation is key for therapeutic efficacy. Host cell type significantly impacts viral vaccine glycan profiles, influencing antigenicity and potency.
Area of Science:
- Biotechnology
- Biopharmaceutical Manufacturing
- Vaccine Development
Background:
- Protein glycosylation is a critical quality attribute affecting therapeutic protein efficacy.
- Glycosylation is influenced by media composition, host cell enzymes, and bioprocess parameters.
- Viral vaccine glycan profiles are complex and historically difficult to control.
Purpose of the Study:
- To investigate factors influencing protein glycosylation, particularly in viral vaccines.
- To understand the impact of host cell type on viral vaccine glycosylation.
- To highlight the importance of glycan profile analysis for vaccine development and manufacturing.
Main Methods:
- Review of factors affecting cellular glycosylation processes.
- Analysis of glycan profiles in recombinant proteins and viral vaccines.
- Comparison of glycosylation patterns across different host cell types for influenza vaccines.
Main Results:
- Media components, nutrient levels, and bioprocess parameters (pH, oxygen) affect protein glycosylation.
- Host cell type is the primary determinant of viral vaccine glycan profiles.
- Influenza virus hemagglutinin glycosylation impacts vaccine antigenicity and efficacy.
Conclusions:
- Understanding and controlling glycosylation is crucial for consistent production of effective biotherapeutics.
- Host cell selection is paramount for achieving desired glycan profiles in viral vaccines.
- Glycan profile analysis will be increasingly vital for developing safe and potent vaccines.
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