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Updated: Feb 18, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
Analysis of Host-Takeover During SPO1 Infection of Bacillus subtilis
1Department of BioSciences, Rice University, 6100 Main St., Houston, TX, 77251, USA. crs@rice.edu.
Abstract:
When Bacillus subtilis is infected by bacteriophage SPO1, the phage directs the remodeling of the host cell, converting it into a factory for phage reproduction. Much synthesis of host DNA, RNA, and protein is shut off, and cell division is prevented. Here I describe the protocols by which we have demonstrated those processes, and identified the roles played by specific SPO1 gene products in causing those processes.
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