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Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology
Philip Kelleher1, James Murphy1, Jennifer Mahony1,2
1School of Microbiology, University College Cork, Cork, Ireland.
Abstract:
Whole phage genomes can be sequenced readily using one or a combination of next generation sequencing (NGS) technologies. One of the most recently developed NGS platforms, the so-called Single-Molecule Real-Time (SMRT) sequencing approach provided by the PacBio RS platform, is particularly useful in providing complete (i.e., un-gapped) genome sequences, but differs from other technologies in that the platform also allows for downstream analysis to identify nucleotides that have been modified by DNA methylation. Here, we describe the methodological approach for the detection of genomic methylation motifs by means of SMRT sequencing.
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