Determination of multiple antibiotics in leafy vegetables using QuEChERS-UHPLC-MS/MS
Xiaolu Yu1, Hang Liu1, Chengjun Pu1
1Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Science, China Agricultural University, Beijing, P. R. China.
Journal of Separation Science
|November 15, 2017
Summary
A new method efficiently extracts multiple antibiotic residues from leafy vegetables. This analysis ensures food safety by detecting contaminants, with recoveries ranging from 57-91%.
Area of Science:
- Food Science
- Analytical Chemistry
- Environmental Science
Background:
- Antibiotic residues in vegetables pose risks to human health.
- Accurate detection methods are crucial for food safety monitoring.
Purpose of the Study:
- To develop a simultaneous extraction and cleanup method for multiple antibiotics in leafy vegetables.
- To analyze antibiotic residues using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
Main Methods:
- A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) approach was employed.
- Extraction utilized citric acid/sodium citrate in acetonitrile/methanol solvent.
- Octadecylsilyl and graphitized carbon black were used as dispersant adsorbents.
Main Results:
- The method effectively extracted 20 target antibiotics from leafy vegetables.
- Average recoveries for the antibiotics ranged from 57% to 91%.
- Limits of detection were between 0.33-2.92 μg/kg, demonstrating high sensitivity.
Conclusions:
- The developed UHPLC-MS/MS method is selective, sensitive, and reliable for detecting multiple antibiotic residues.
- Establishing standards for antibiotic residues in vegetables is recommended to ensure food safety.
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