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HLA-B27 Correlates with the Intracellular Elimination, Replication, and Trafficking of Salmonella Enteritidis
Yi Zhao1, Honghu Tang1, Chunyu Tan1
1Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan, China (mainland).
Abstract:
BACKGROUND The aim of this study was to explore the correlation between HLA-B27 and the intracellular elimination, replication, and trafficking of Salmonella enteritidis (S. enteritidis) collected from patients with reactive arthritis. MATERIAL AND METHODS Confocal microscopy, flow cytometry, and sandwich enzyme-linked immunosorbent assay (ELISA) were employed in this study to evaluate the localization of proteins of interest, to assess the intracellular trafficking of S. enteritidis, and to measure the production of cytokines of interest. RESULTS HLA-B27 was negatively associated with intracellular S. enteritidis elimination in healthy human monocytes/macrophages. In S. enteritidis infected monocytes/macrophages, HLA-27B was also negatively correlated with bacteria elimination but positively related to bacteria replication. S. enteritidis did not co-localize with NRAMP1 and LAMP1/2 in HLA-B27 cells. S. enteritidis did not co-exist with transferrin or dextran within HLA-B27 and A2 cells. CONCLUSIONS HLA-B27 is closely associated with the intracellular elimination and replication of S. enteritidis. Replicated bacteria in HLA-B27 monocytic cells were located within unique vacuoles rather than disturbing host endocytosis.
Insights
Human Leukocyte Antigen B27 (HLA-B27) is linked to reduced Salmonella enteritidis elimination and increased bacterial replication in monocytes. This suggests HLA-B27 influences bacterial handling within immune cells.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Reactive arthritis is associated with Human Leukocyte Antigen B27 (HLA-B27).
- Salmonella enteritidis (S. enteritidis) infection can trigger reactive arthritis.
- The role of HLA-B27 in the host's response to S. enteritidis is not fully understood.
Purpose of the Study:
- To investigate the correlation between HLA-B27 and the intracellular fate of S. enteritidis.
- To examine how HLA-B27 affects bacterial elimination, replication, and trafficking within host cells.
Main Methods:
- Confocal microscopy and flow cytometry were used to assess protein localization and bacterial trafficking.
- Sandwich enzyme-linked immunosorbent assay (ELISA) measured cytokine production.
- Monocytes/macrophages from patients and healthy individuals were utilized.
Main Results:
- HLA-B27 showed a negative association with intracellular S. enteritidis elimination in monocytes/macrophages.
- HLA-B27 was positively correlated with S. enteritidis replication within infected cells.
- S. enteritidis did not co-localize with NRAMP1, LAMP1/2, transferrin, or dextran in HLA-B27 expressing cells, indicating unique vacuolar compartments.
Conclusions:
- HLA-B27 is significantly associated with impaired intracellular elimination and enhanced replication of S. enteritidis.
- Replicated S. enteritidis resides in distinct vacuoles within HLA-B27 positive monocytic cells, bypassing typical endocytic pathways.
